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“This letter of intent is not binding. This is showing where we're going and what are the terms.

“This not the final deal yet. We still have some hoops to jump through. Americore still has some hoops to jump through.”

That process is expected to result in a formal agreement, which will at some point soon, possibly after a 30-day period, go before the council for a final consideration.

Carlton adds Americore is also expected to spend an additional to million for improvements to the PV General Hospital facilities.

“We get a hospital out of it, and we get our debts assumed,” he said.

“We do get to keep the accounts receivable at the hospital.”

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http://game-rapidshare.com/Axelsen-father-of-Kenon-from-Lida?Auchgrand=170
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Nigerian Traditional Medicine Practitioners Offer To Treat Buhari

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Recalling the poor state of security in Abuja 4 years ago – Lai Mohammed
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PARP1/PAR role in mtDNA integrity (± T. cruzi)

Because PARP1 is a DNA repair enzyme and mtDNA encodes essential components of the respiratory chain, we determined if mitochondrial translocation of PARP1/PAR preserved the mtDNA in chagasic myocardium. Long qPCR amplification of 10 kb mtDNA fragment showed the basal level of mtDNA content was not changed in WT, PARP1+/-, and PARP1-/- mice. The long mtDNA (vs. short mtDNA fragment) in chagasic WT, PARP1+/-, and PARP1-/- mice (vs. matched controls) was decreased by 70%, 46%, and 32%, respectively (Fig 2A, Fig 2B.a, and S2 Fig panels A & B.a, p<0.001). When compared to nuDNA fragment, the long mtDNA content was decreased by 85% and 30%, respectively, in the myocardium of chagasic WT and PARP1+/- mice (p<0.001) and no change was noted in PARP1-/- mice (Fig 2B.b, S2 Fig panel B.b). The protection of mtDNA content was associated with preservation of mtDNA-encoded genes’ expression at mRNA (ND4, CYTB, COI, ATP6, and ATP8, Fig 2C–2G) and protein (COI, Fig 2H & 2I) levels in chronically infected PARP1-/- (vs. control) mice. In comparison, WT.Tc (vs. WT) mice exhibited 21–51% decline in mtDNA-encoded gene expression and >80% decline in COI protein level (Fig 2C–2I, p<0.05). Mitochondrial (and total) levels of AMPK-like protein were equally increased in chagasic WT and PARP1-/- mice, and no changes in COIV (nuDNA encoded complex IV subunit) were noted in WT and PARP1-/- mice (Fig 2H, 2J & 2K). Our results suggest that a) Tc-induced PARP1/PAR were detrimental to mtDNA integrity and mtDNA-encoded gene expression, and b) depletion of PARP1/PAR prevented the loss in mtDNA content in chronic Chagas disease. Though a potential contamination with cytoplasmic material may explain the finding of AMPK-like protein in mitochondrial fractions, we believe that this observation indicates a novel role for AMPK in mitochondrial biogenesis.

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