5 Star
.

Comments

We encourage all readers to share their views on our articles and blog posts. We are committed to maintaining a lively but civil forum for discussion, so we ask you to avoid personal attacks, and please keep your comments relevant and respectful. If you encounter a comment that is abusive, click the "X" in the upper right corner of the comment box to report spam or abuse. We are using Facebook commenting. Visit our FAQ page for more information. .
Fig 5. Treatment with PARP1 inhibitor improved mitochondrial biogenesis in chagasic mice.

C57BL/6 mice were infected with T. cruzi, treated with PJ34 (12.5 mg/100-?l/mouse, intraperitoneally, twice a week for three weeks beginning at 45 days’ pi, and sacrificed at 150 days’ pi. (A) RT-qPCR evaluation of myocardial level of PARP1 mRNA, normalized to GAPDH mRNA (n ? 5 mice/group, triplicate observations per mouse). (B-E) Representative Western blot images of myocardial level of PARP1 with GAPDH loading control (n = 3 mice/group) and PAR levels in mice treated with increasing concentration of PJ34 (0–25 mg/kg) are shown in B & D, respectively. Densitometry analysis was performed for all Western blot gels from n ? 6 mice/group, and PARP1 and PAR levels (normalized to GAPDH levels) are shown in C & E, respectively. (F&G) Representative gel images (F, n = 3 mice/group) show myocardial levels of 10 kb mtDNA with 177-bp mtDNA and 96-bp GAPDH (nuDNA) fragments as controls. The PCR amplification was performed for 28 cycles. Densitometry analysis was performed on PCR gels representing n ? 6 mice/group, and density of the 10 kb mtDNA band, normalized against mtDNA and nuDNA fragments, is presented (G.a&b). (H-O) Bar graphs (n ? 5 mice/group, duplicate or triplicate observations per sample) show the myocardial (H-K & N) and plasma (L, M & O) levels of H2O2 (H), 3-nitrotyrosine (I), protein carbonyls (J&L), lipid hydroperoxides (K&M) and antioxidant capacity (N&O). (P) Myocardial parasite burden in chronically infected (± PJ34 treatment) mice was determined by qPCR amplification of Tc18SrDNA and normalized with GAPDH (n? 5 mice/group, three observations per mouse). Data in all bar graphs are plotted as mean value ± SEM, and statistical significance are marked as *infected vs. control and #infected/PJ34-treated vs. infected/untreated (*,#p<0.05, **,##p<0.01, ***,###p<0.01).

https://doi.org/10.1371/journal.ppat.1007065.g005

.
.
.
http://game-rapidshare.com/Fambrough-father-of-Marohnic-from-Wotersen?Behrensgr=247
.
.
.
Books
  • .
    ..
    .