(A) Mice (WT, PARP1+/-, and PARP1-/-) were infected with Tc and sacrificed at 150 days’ pi. (A-F) Shown is RT-qPCR evaluation of myocardial levels of mRNAs for components of the mtDNA replication and transcription machinery in chronically infected and control mice. Data were normalized to GAPDH mRNA (n ? 5 mice/group, triplicate observations per mouse). (G-I) Heart homogenates were subjected to differential centrifugation to isolate mitochondria. Representative Western blot images for cardiac mitochondrial levels of POLG (G, n = 4 mice/group) and other proteins of mtDNA replication machinery (I) in WT and PARP1-/- mice are shown (loading control: COIV). Densitometry analysis was performed for Western blot gels representing n? 4 mice/group, and data were normalized to COIV levels (H). (J) Cardiac mitochondria from normal and chagasic WT mice (n = 5/group) were pooled and subjected to immunoprecipitation with anti-POLG antibody. Input (total), flow-through (unbinding), and POLG-binding fractions were used for Western blotting with antibodies against PARP1, Twinkle, and SSBP1. WB with anti-POLG antibody was performed to confirm high efficiency of POLG pull down by immunoprecipitation in all samples. The gel images are representative of three independent experiments. (K&L) PARP1-POLG binding and POLG activity in Tc-infected cells. HeLa cells were infected with T. cruzi (cell: parasite ratio, 1:5) for 24 h (controls: uninfected or treated with 50 ?M H2O2). Cells were used for cross-IP/WB with antibodies against POLG and PARP1 (K). HeLa cells (± T. cruzi) were treated with formaldehyde to cross-link proteins and DNA. After immunoprecipitation with PARP1 antibody, DNA was used for PCR amplification of mitochondrial 7SDNA and nuclear COIV DNA fragments. The GAPDH and 7S DNA in input samples were amplified to confirm equal loading of all samples (L). Data in K and L are representative of three biological replicates. Data in bar graphs are plotted as mean value ± SEM, and statistical significance are marked as *WT.Tc vs. WT, &PARP1-/-.Tc vs. PARP1-/-, and #WT.Tc vs. PARP1-/-.Tc (*,&,#p<0.05, **p<0.01).
(KUTV) - The John A. Moran Eye Center at the University of Utah reported Saturday that it will be notifying 607 patients of a potential disclosure of some of their information following a theft of electronic equipment.
A laptop computer and external storage device (used to take and store retinal images) were stolen from a locked storage unite at 65 Mario Capecchi Drive in Salt Lake City. The eye center learned about this theft on April 3, 2018.
The devices contained retinal images, full or partial name and dates of birth for patients and medical reference numbers for 602 infants and 5 adults, all of whom had images taken by Moran specialists conducting evaluations at the University of Utah Hospital and Primary Children's Hospital between July 1, 2014 and March 30, 2018.
No social security numbers or financials records were stored on either device. This investigation is still ongoing.
"As part of University of Utah Health, the John A. Moran Eye Center is fully committed to protecting the privacy of our patients," Randall J. Olson, CEO of the John A. Moran Eye Center, said in a statement. "I sincerely regret that personal information about any of our patients is ever exposed and especially so for children. While no financial information was disclosed, I understand the concerns that impacted patients and parents may have. For peace of mind, we are offering free credit monitoring for any child or adult whose data may have been compromised."
The Moran Eye Center is working to improve its policy and procedures and enhance security measures to reduce the risk of an event like this from happening again.
"Patient trust is fundamental to everything we do at the Moran Eye Center, and our team is conducting a comprehensive review of our policies, procedures, and security measures to ensure patient information is always protected," Olson said.
Impacted patients and parents or guardians will receive letters by U.S. mail. If you are an impacted individuals and you would like to ask a question about this situation, please call 855-349-6456 between 7 a.m. and 7 p.m. MDT Monday through Friday.
Mice were infected with T. cruzi (10,000 parasites per mouse). Vevo 2100 ultrasound system was used to perform transthoracic echocardiography in B and M mode and pulse-wave Doppler (PWD) echocardiography to assess the left ventricular and mitral valve functions at 150 days’ post-infection (n = 8–12 mice per group, three recordings per mouse). Data are presented as mean value ± SEM. Statistical significance is plotted as * (normal vs. infected) and # (PARP1+/+.Tc vs. PARP1+/- .Tc or PARP1-/-.Tc) and presented as *,# <0.05, **,## p<0.01, ***,### p<0.001.