5 Star
.
.
.

Osinbajo vows never to stab Buhari in the back 0

.

S1 Fig.

(A) Genotyping of mice. Total DNA was isolated from tail biopsies, and it was utilized as template in a PCR reaction with following oligonucleotides: Forward, 5’-CATGTTCGATGGGAAAGTCCC-3’; Reverse 1, 5’-CCAGCGCAGCTCAGAGAAGCCA-3’; and Reverse 2: 5’-AGGTGAGATGACAGGAGATC-3’. The F1/R1 oligonucleotides amplified a 112 bp band in WT (PARP1+/+) mice, and F1/R2 oligonucleotides amplified a 350 bp band in PARP1-/- mice. Amplification of both bands indicated PARP1+/- genotype. (B) Confirmation of mitochondrial fractions purity by PCR. Mitochondria Isolation Kit for Tissue (Abcam110168) was employed to isolate mitochondrial fractions. Total DNA from mitochondrial fractions was utilized as template in traditional PCR with gene-specific oligonucleotides to amplify for 28 cycles the 7S mtDNA fragment (184 bp) and GAPDH nuDNA fragment (101 bp). The PCR amplicons were resolved on 1.5% agrose gel. Note the amplification of nuDNA fragment was not noted in mitochondrial fractions.

https://doi.org/10.1371/journal.ppat.1007065.s003

(TIF)

http://game-rapidshare.com/Difabio-son-of-Visnocky-from-Olifants-Kop?Deschepper=101

Independent News Email

Please enter an email address Email address is invalid Fill out this field Email address is invalid Email cannot be used. Try another .
.
.
.
.
.
.
.

5 Star All Rights Reserved. Star pulse 2018