WT, PARP1+/-, and PARP1-/- mice were monitored at 150 days’ post-infection. Shown are transthoracic echocardiography measurements of (A) stroke volume (SV), (B) cardiac output (CO), and (C) ejection fraction (EF). Pulse-wave doppler echocardiography was performed to measure (D) isovolumic contraction time (IVCT), (E) LV ejection time, mitral valve (F) early and (G) late peak velocities, and (H) isovolumic relaxation time (IVRT). The data presented in A-H were acquired from n = 8–12 mice/group with triplicate recordings per mouse. (I) Myocardial parasite burden was determined by qPCR amplification of Tc18SrDNA and normalized with GAPDH (n? 5 mice/group, three observations per mouse). Data in all bar graphs are plotted as mean value ± SEM. Statistical significance are marked as *WT.Tc vs. WT, &genetically modified/infected vs. matched controls, and #WT.Tc vs. genetically-modified/infected (*,&,#p<0.05, **,##p<0.01). Detailed LV function data are presented in S2 Table.