WT, PARP1+/-, and PARP1-/- mice were monitored at 150 days’ post-infection. Shown are transthoracic echocardiography measurements of (A) stroke volume (SV), (B) cardiac output (CO), and (C) ejection fraction (EF). Pulse-wave doppler echocardiography was performed to measure (D) isovolumic contraction time (IVCT), (E) LV ejection time, mitral valve (F) early and (G) late peak velocities, and (H) isovolumic relaxation time (IVRT). The data presented in A-H were acquired from n = 8–12 mice/group with triplicate recordings per mouse. (I) Myocardial parasite burden was determined by qPCR amplification of Tc18SrDNA and normalized with GAPDH (n? 5 mice/group, three observations per mouse). Data in all bar graphs are plotted as mean value ± SEM. Statistical significance are marked as *WT.Tc vs. WT, &genetically modified/infected vs. matched controls, and #WT.Tc vs. genetically-modified/infected (*,&,#p<0.05, **,##p<0.01). Detailed LV function data are presented in S2 Table.
Finally, we obtained a quantitative measure of tissue parasite burden to confirm if the observed benefits of PARP1 depletion are delivered through its effects on parasite persistence. Myocardial level of Tc18SrDNA were similarly increased in chagasic WT, PARP1+/-, and PARP1-/- mice (Fig 7I). Together, the results presented in Fig 6 and Fig 7 suggest that changes in the LV walls’ thickness (and thereby contractile capacity) contributed to compromised systolic and diastolic performance of the heart in chagasic WT mice. The benefits of PARP1 depletion in preserving LV hemodynamics and myocardial performance were delivered via control of collagenosis and stiffness of IVS and LVPW in the myocardium of chagasic mice..
Juul’s team tried it in a group of 20 patients. Nine got poop transplants at the first sign of C. diff-related diarrhea, and 11 got the antibiotic metronidazole.
Five out of nine who got the fecal transplant were cured by a single transplant delivered by enema, and five of the 11 who got antibiotics were cured, the team reported. Their findings are being presented and discussed at a meeting of gastroenterologists called Digestive Disease Week.
Four days after the fecal transplant, three of the patients who still had diarrhea got antibiotics and two of them were cured. But giving more antibiotics to the six patients who got drugs to start with did not help them recover, the researchers said.
”The targets to end AIDS stipulates that 90 per cent of persons living with HIV would know their status by 2020 and 90 per cent of those diagnosed with HIV would receive antiretroviral therapy and 90 per cent of those receiving treatment would be virally suppressed.
Moma-Efretuei said that this imitative would form the bedrock on which future interventions would lie.
The FCT Regional Manager, Institute of Human Virology, Nigeria, Dr Olayemi Olupitan, urged the Nigerian government to intensify efforts and awareness on HIV testing and treatment.
Olupitan added that UNAIDS had reached advanced stage in its trategy to end AIDS epidemic by 2030 in Nigeria
She added that the Institute of Human Virology, Nigeria, was collaborating with the National Assembly and Methodist Church to provide free medical services to the residents of Karimajiji IDPs.
The Chairman, Karimajiji IDP camp, Mohammed Abubarka, called on the Federal Government to support the IDPs to return to their homes.
Abubarka said that the suffering was too much and it would be appreciated if they could go back home.
”We want to go back home, we want to go back to farming and working, there is no place like home, we are not happy here.
”We have no food, hospital or school, we are suffering and we need help, we have been here for over three years,” he said..
Kari Paul is a personal finance reporter based in New York. You can follow her on Twitter @kari_paul..