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Histology

Tissue sections were fixed in 10% buffered formalin, dehydrated in absolute ethanol, cleared in xylene, and embedded in paraffin. Five-micron tissue sections were subjected to Masson’s Trichrome staining at the Research Histopathology Core at the UTMB, and fibrosis was assessed by measuring the collagen area as a percentage of the total myocardial area (n = 4 mice /group, 2–3 slides per mouse, 10 microscopic fields per slide) using Simple PCI software (v.6.0; Compix, Sewickley PA). Sections were scored based on percent of fibrotic area: (0) <1%, (1) 1–5%, (2) 5–10%, (3) 10–15%, and (4) >15% [24].

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