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Celia Hoffman | 02 June, 2018, 03:04 .

“This letter of intent is not binding. This is showing where we're going and what are the terms.

“This not the final deal yet. We still have some hoops to jump through. Americore still has some hoops to jump through.”

That process is expected to result in a formal agreement, which will at some point soon, possibly after a 30-day period, go before the council for a final consideration.

Carlton adds Americore is also expected to spend an additional to million for improvements to the PV General Hospital facilities.

“We get a hospital out of it, and we get our debts assumed,” he said.

“We do get to keep the accounts receivable at the hospital.”

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Fig 2. Mitochondrial DNA integrity and expression of mtDNA-encoded genes in chagasic mice (± PARP1).

Mice (WT and PARP1-/-) were infected with T. cruzi and sacrificed at 150 days’ pi. (A) Representative gel images (n = 3 mice/group) show myocardial levels of 10 kb mtDNA with short 177-bp mtDNA and 96-bp GAPDH (nuDNA) fragments as controls. The PCR amplification was conducted for 28 cycles. (B) Densitometry analysis was performed on PCR gels representing n? 6 mice/group, and density of the 10 kb mtDNA band was normalized against mtDNA (B.a) and nuDNA (B.b) fragments. (C-G) Myocardial mRNA levels for mtDNA-encoded genes were determined by RT-qPCR, and normalized to GAPDH mRNA (n ? 5, triplicate observations per mouse). (H-K) Representative Western blotting images (n = 3 mice per group) of cardiac mitochondrial levels of COI and AMPK-like (COIV, loading control) and total heart homogenate levels of AMPK (GAPDH, loading control) are shown (H). Densitometry analysis was performed on gel images for n = 6 mice for each protein, and data for COI and AMPK-like levels in mitochondrial fractions (I&J) and AMPK levels in total heart homogenates (K) were normalized to COIV and GAPDH, respectively. In bar graphs, data are plotted as mean value ± SEM. Statistical significance are marked as *WT.Tc vs. WT, &PARP1-/-.Tc vs. PARP1-/-, and # WT.Tc vs. PARP1-/-.Tc (*,#p<0.05, ***,&&&,###p<0.001).

https://doi.org/10.1371/journal.ppat.1007065.g002

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  • Trypanosoma cruzi