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S1 Fig.

(A) Genotyping of mice. Total DNA was isolated from tail biopsies, and it was utilized as template in a PCR reaction with following oligonucleotides: Forward, 5’-CATGTTCGATGGGAAAGTCCC-3’; Reverse 1, 5’-CCAGCGCAGCTCAGAGAAGCCA-3’; and Reverse 2: 5’-AGGTGAGATGACAGGAGATC-3’. The F1/R1 oligonucleotides amplified a 112 bp band in WT (PARP1+/+) mice, and F1/R2 oligonucleotides amplified a 350 bp band in PARP1-/- mice. Amplification of both bands indicated PARP1+/- genotype. (B) Confirmation of mitochondrial fractions purity by PCR. Mitochondria Isolation Kit for Tissue (Abcam110168) was employed to isolate mitochondrial fractions. Total DNA from mitochondrial fractions was utilized as template in traditional PCR with gene-specific oligonucleotides to amplify for 28 cycles the 7S mtDNA fragment (184 bp) and GAPDH nuDNA fragment (101 bp). The PCR amplicons were resolved on 1.5% agrose gel. Note the amplification of nuDNA fragment was not noted in mitochondrial fractions.

https://doi.org/10.1371/journal.ppat.1007065.s003

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Motorsports .

Morning commute got you down? Consider moving to Vermont — you may make ,000 out of it.

The state’s governor signed a law Wednesday that will give workers up to ,000 in tax credits to move there through its New Remote Worker Grant Program. The legislation, which goes into effect Jan. 1, is part of a larger effort to augment the state’s declining and aging population and draw more people from the tech workforce there.

In addition to the grant program, Vermont has a statewide policy on flexible working arrangements — a statute meant to protect residents’ right to request flexible working arrangements without fear of retaliation.

Employees aren’t the only ones Vermont is trying to draw to its rolling hills: Companies that move to Vermont can get up to 5,000 in total credits in 2019 and up to 0,000 in 2020 to cover relocation costs, equipment expenses, broadband updates and employee membership fees for co-working spaces.

More companies across the country are allowing employees flexibility, according to Kareem Bakr, director at the global recruitment organization Selby Jennings. Some 50% of companies the firm works with offer at least one day of remote work per year, and 25% allow employees to work full-time from wherever they want outside the office.

“They are becoming more comfortable with employees working remotely because it allows them to tap into different talent pools,” he said. “The biggest incentive is work-life balance.”

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