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Sears, 1 Kmart stores in N.J. set to close

Five Illinois stores were on the list , including a Kmart in Rockford and Sears stores in Vernon Hills, Gurnee and Springfield. The company said the full list of stores that would be closing will be released later in the day on Thursday.
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Trump starts trade war with allies, Europe to respond 'within hours'

Canada has promised tariffs on US exports of steel, aluminum and other goods, to match the USA tariffs "dollar for dollar". Britain exports around ?360m in steel to the United States every year, 10% of the industry's total exports.
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First Amendment: Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof; or abridging the freedom of speech, or of the press; or the right of the people peaceably to assemble, and to petition the Government for a redress of grievances.

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Chelsea put stadium plans on hold amid Abramovich visa woes ..

In-tissue mitochondrial function analysis

Freshly harvested tissues (~10 mg) were immersed in ice-cold BIOPS buffer (10 mM CaK2-EGTA, 7.2 mM K2-EGTA, 20 mM imidazole, 20 mM taurine, 50 mM K-MES, 0.5 mM dithiothreitol, 6.5 mM MgCl2, 5.8 mM ATP, and 15 mM creatine phosphate; pH 7.1). Myofiber bundles were transferred to 2 ml of MIR05 buffer (0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH2PO4, 20 mM HEPES, 110 mM sucrose, and 1 mg/ml fatty acid free bovine serum albumin, pH 7.1) containing 50-?g/ml saponin, and incubated at 4°C for 30 min to achieve chemical permeabilization of the sarcolemma membrane. Permeabilized myofiber bundles (~2 mg) were washed with MIR05 buffer, and used for measuring mitochondrial respiration by using an Oxygraph-2k (O2K) respirometer (Oroboros Instruments, Innsbruck Austria). Oxygen concentration was determined at 2 sec intervals and used to compute oxygen flux per mg of tissue by Oroboros DatLab software. Briefly, after recording the baseline respiration with myofiber bundles alone, 5 mM pyruvate, 2 mM malate and 10 mM glutamate (P+G+M) were added, and complex I driven state 4 respiration was recorded. Electron transfer was coupled to phosphorylation by the addition of 5 mM ADP, and state 3 respiration was recorded. Maximal state 3 respiration with parallel electron input from complex I and complex II was recorded with addition of 10 mM succinate, and complex II supported respiration was measured in presence of 6.25 ?M rotenone (inhibits complex I) [22].

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