5 Star
Image: Clostridium difficile
Clostridium difficile. A new study shows treating patients straight away with fecal transplants might clear up the infection more quickly than using antibiotics to start with.CDC



Sign up to continue reading and gain Free Access to all our resources.

Sign up for our free newsletter and join 60,000 of
your peers to stay up to date with tips and advice on:

EHR Optimization
EHR Interoperability
EHR Replacement

White Papers, Webcasts, Featured Articles and Exclusive Interviews

Our privacy policy

Fig 5. Treatment with PARP1 inhibitor improved mitochondrial biogenesis in chagasic mice.

C57BL/6 mice were infected with T. cruzi, treated with PJ34 (12.5 mg/100-?l/mouse, intraperitoneally, twice a week for three weeks beginning at 45 days’ pi, and sacrificed at 150 days’ pi. (A) RT-qPCR evaluation of myocardial level of PARP1 mRNA, normalized to GAPDH mRNA (n ? 5 mice/group, triplicate observations per mouse). (B-E) Representative Western blot images of myocardial level of PARP1 with GAPDH loading control (n = 3 mice/group) and PAR levels in mice treated with increasing concentration of PJ34 (0–25 mg/kg) are shown in B & D, respectively. Densitometry analysis was performed for all Western blot gels from n ? 6 mice/group, and PARP1 and PAR levels (normalized to GAPDH levels) are shown in C & E, respectively. (F&G) Representative gel images (F, n = 3 mice/group) show myocardial levels of 10 kb mtDNA with 177-bp mtDNA and 96-bp GAPDH (nuDNA) fragments as controls. The PCR amplification was performed for 28 cycles. Densitometry analysis was performed on PCR gels representing n ? 6 mice/group, and density of the 10 kb mtDNA band, normalized against mtDNA and nuDNA fragments, is presented (G.a&b). (H-O) Bar graphs (n ? 5 mice/group, duplicate or triplicate observations per sample) show the myocardial (H-K & N) and plasma (L, M & O) levels of H2O2 (H), 3-nitrotyrosine (I), protein carbonyls (J&L), lipid hydroperoxides (K&M) and antioxidant capacity (N&O). (P) Myocardial parasite burden in chronically infected (± PJ34 treatment) mice was determined by qPCR amplification of Tc18SrDNA and normalized with GAPDH (n? 5 mice/group, three observations per mouse). Data in all bar graphs are plotted as mean value ± SEM, and statistical significance are marked as *infected vs. control and #infected/PJ34-treated vs. infected/untreated (*,#p<0.05, **,##p<0.01, ***,###p<0.01).