Fig 2. Mitochondrial DNA integrity and expression of mtDNA-encoded genes in chagasic mice (± PARP1).
Mice (WT and PARP1-/-) were infected with T. cruzi and sacrificed at 150 days’ pi. (A) Representative gel images (n = 3 mice/group) show myocardial levels of 10 kb mtDNA with short 177-bp mtDNA and 96-bp GAPDH (nuDNA) fragments as controls. The PCR amplification was conducted for 28 cycles. (B) Densitometry analysis was performed on PCR gels representing n? 6 mice/group, and density of the 10 kb mtDNA band was normalized against mtDNA (B.a) and nuDNA (B.b) fragments. (C-G) Myocardial mRNA levels for mtDNA-encoded genes were determined by RT-qPCR, and normalized to GAPDH mRNA (n ? 5, triplicate observations per mouse). (H-K) Representative Western blotting images (n = 3 mice per group) of cardiac mitochondrial levels of COI and AMPK-like (COIV, loading control) and total heart homogenate levels of AMPK (GAPDH, loading control) are shown (H). Densitometry analysis was performed on gel images for n = 6 mice for each protein, and data for COI and AMPK-like levels in mitochondrial fractions (I&J) and AMPK levels in total heart homogenates (K) were normalized to COIV and GAPDH, respectively. In bar graphs, data are plotted as mean value ± SEM. Statistical significance are marked as *WT.Tc vs. WT, &PARP1-/-.Tc vs. PARP1-/-, and # WT.Tc vs. PARP1-/-.Tc (*,#p<0.05, ***,&&&,###p<0.001).
Morning commute got you down? Consider moving to Vermont — you may make ,000 out of it.
The state’s governor signed a law Wednesday that will give workers up to ,000 in tax credits to move there through its New Remote Worker Grant Program. The legislation, which goes into effect Jan. 1, is part of a larger effort to augment the state’s declining and aging population and draw more people from the tech workforce there.
In addition to the grant program, Vermont has a statewide policy on flexible working arrangements — a statute meant to protect residents’ right to request flexible working arrangements without fear of retaliation.
Employees aren’t the only ones Vermont is trying to draw to its rolling hills: Companies that move to Vermont can get up to 5,000 in total credits in 2019 and up to 0,000 in 2020 to cover relocation costs, equipment expenses, broadband updates and employee membership fees for co-working spaces.
More companies across the country are allowing employees flexibility, according to Kareem Bakr, director at the global recruitment organization Selby Jennings. Some 50% of companies the firm works with offer at least one day of remote work per year, and 25% allow employees to work full-time from wherever they want outside the office.
“They are becoming more comfortable with employees working remotely because it allows them to tap into different talent pools,” he said. “The biggest incentive is work-life balance.”