“Vital prevention efforts such as tobacco control, dietary interventions, and broader health promotion campaigns need to be scaled up in response to this rise in lifestyle-related cancers.”
The rise in lifestyle cancers has been offset in part by a drop in cervical and stomach cancers, which are caused by infectious diseases like the human papillomavirus..
Echocardiography assessment of LV structure and function
Mice were continuously anesthetized by inhalant 1.5% isoflurane/100% O2 to maintain a light sedation level. Mice were placed supine on an electrical heating pad at 37°C during the examination. The echocardiography (ECG) electrodes of the Vevo 2100 ultrasound system (Visual Sonics, Toronto, Canada) were connected to mouse paws, and heart rate and respiratory physiology were continuously monitored. Mice chests were shaved, and warmed ultrasound gel was applied to the area of interest. Transthoracic echocardiography was performed using the high-frequency linear array transducer (MS400, 18–38 MHz) of the Vevo 2100 ultrasound system. Heart was imaged in B-mode and M-mode to examine the parameters of left ventricle (LV) in diastole (-d) and systole (-s). Pulse wave Doppler imaging was performed to measure heart diastolic function. All measurements were obtained in triplicate and data were analyzed by using Vevo 2100 standard measurement software .