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Trump’s summit with North Korea’s Kim Jong Un is now back on

The letter: The Wall Street Journal , quoting a foreign government official, said the letter from Kim Jong Un was "fairly basic". Trump had a two-hour meeting with an envoy for the North Korean regime, right-hand man to Kim Jong-un, Kim Yong Chol .
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Echocardiography assessment of LV structure and function

Mice were continuously anesthetized by inhalant 1.5% isoflurane/100% O2 to maintain a light sedation level. Mice were placed supine on an electrical heating pad at 37°C during the examination. The echocardiography (ECG) electrodes of the Vevo 2100 ultrasound system (Visual Sonics, Toronto, Canada) were connected to mouse paws, and heart rate and respiratory physiology were continuously monitored. Mice chests were shaved, and warmed ultrasound gel was applied to the area of interest. Transthoracic echocardiography was performed using the high-frequency linear array transducer (MS400, 18–38 MHz) of the Vevo 2100 ultrasound system. Heart was imaged in B-mode and M-mode to examine the parameters of left ventricle (LV) in diastole (-d) and systole (-s). Pulse wave Doppler imaging was performed to measure heart diastolic function. All measurements were obtained in triplicate and data were analyzed by using Vevo 2100 standard measurement software [13].

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